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| Bompard, G.; Rabeharivelo, G.; Cau, J.; Abrieu, A.; Delsert, Claude; Morin, N.. |
| The oncogenic kinase PAK4 was recently found to be involved in the regulation of the G1 phase and the G2/M transition of the cell cycle. We have also identified that PAK4 regulates Ran GTPase activity during mitosis. Here, we show that after entering mitosis, PAK4-depleted cells maintain a prolonged metaphase-like state. In these cells, chromosome congression to the metaphase plate occurs with normal kinetics but is followed by an extended period during which membrane blebbing and spindle rotation are observed. These bipolar PAK4-depleted metaphase-like spindles have a defective astral microtubule MT network and are not centered in the cell but are in close contact with the cell cortex. As the metaphase-like state persists, centrosome fragmentation occurs,... |
| Tipo: Text |
Palavras-chave: P21-activated kinase 4; Metaphase; Spindle orientation; Spindle assembly checkpoint. |
| Ano: 2013 |
URL: http://archimer.ifremer.fr/doc/00133/24465/22502.pdf |
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